RESUMO
Objective:To explore the changing characteristics of interpretive bias of senior high school students with different levels of autistic traits in ambiguous situations, and to investigate the consistency of interpretive bias across self/other relevant conditions.Methods:A survey of 1 080 senior high school students from a high school in Anhui province was conducted by using the autistic-spectrum quotient (AQ). According to the criteria of high scores and low scores of 27%, the subjects in the high autistic trait group ( n=266) and the low autistic trait group ( n=266) were selected.The ambiguous situation paradigm was used to measure the frequency of positive interpretation of ambiguous information by two groups of subjects under the conditions of self and others, SPSS 26.0 was used for descriptive statistics, and jamovi 2.2.3 was used for generalized linear mixed model analysis. Results:(1) The results of the between-group effects at the level of autistic traits indicated that the frequency of positive interpretations in the high autistic group was significantly lower than that in the low autistic group(54.5(11.8), 57(11.8) )( χ2=13.99, P<0.001). The results of the interaction between level of autistic traits and type of ambiguous situation indicated that in the positive ambiguous situation, the frequency of positive interpretations in the high autistic group was smaller than that in the low autistic group (33(4), 34(3)) ( P<0.001). There was no significant differences in the number of positive interpretations between high autistic group and low autistic group in negtive ambiguous situation( P>0.05). (2) The results of the main effects of the ambiguous situation types indicated that the frequency of positive interpretations in positive ambiguous situations was significantly higher than that in negative ambiguous situations(33(4), 23(9.25)) ( χ2=1 348.50, P<0.001). The results of the interaction between level of autistic traits and type of ambiguous situation indicated that the frequency of positive interpretations in positive ambiguous situations (33(4), 34(3)) in both high and low autistic groups was larger than that in negative ambiguous situations (22(10), 24(9)) ( P<0.001). (3) The main effects results for the self/other related conditions suggested that the frequency of positive interpretations of familiarity with others (19(4)) was significantly higher than that of self-related conditions (19(5)) and strangers (19(5)) ( χ2=9.51, P<0.01), and there was no significant difference between self-related conditions and strangers( P>0.05). The results of the interaction between ambiguous situation type and self/other related conditions suggested that in the positive ambiguous situation, the frequency of positive interpretations of familiarity with other people's conditions was greater than that of self-related conditions( P<0.01), and in the negative ambiguous situations, there was no significant difference in the frequency of positive interpretations related to different self/others( P>0.05). Conclusions:(1) High school students with high and low autistic traits are more inclined to show positive interpretations in the ambiguous situations, and it higher under the condition of positive ambiguous situations and acquaintances.(2) Compared with those with low autistic traits, individuals with high autistic traits tend to give less positive interpretations to ambiguous situations, but this difference is mainly manifested in positive ambiguous situations.(3) In the negative ambiguous situation, there is no significant difference in number of positive interpretations produced by senior high school students with high and low autistic traits, and they are stable across self and other related conditions.
RESUMO
Objective:To explore the influence of sub-threshold autistic traits on social avoidance and distress of senior high school freshmen, as well as the mediating effect of perspective taking, and to investigate the intervention effect of perspective taking intervention project on improving the ability of perspective taking and reducing social avoidance and distress behavior of high sub-threshold autistic traits individuals.Methods:A total of 1 401 subjects were investigated with the autism-spectrum quotient (AQ), interpersonal reactivity index-C (IRI-C) and social avoidance and distress (SAD) from September 1st to September 30th, 2018. According to the screening criteria of 27% statistically, totally 378 subjects were selected according to the scores of autistic spectrum quotient scale from high to low as the high sub-threshold autistic traits group. The top 32 subjects with high scores of autistic spectrum quotient scale were selected and randomly divided into intervention group and control group with 16 subjects in each group. The modified viewpoint selection group counseling program was used to conduct intervention research on 16 subjects with high sub-threshold autistic traits. SPSS 26.0 was used for common method deviation test, descriptive analysis, correlation analysis and repeated measurement variance analysis, and SPSS 26.0 PROCESS plug-in was used for intermediary effect analysis.Results:(1) The scores of sub-threshold autistic traits, perspective taking, social avoidance and distress of freshmen in senior high school were (113.26±11.35), (12.34±3.95) and (10.84±6.42) respectively. (2) Sub-threshold autistic traits, perspective taking, social avoidance and distress were significantly correlated with each other( r=-0.11-0.52, all P<0.01). (3) Sub-threshold autistic traits had a significant direct effect on social avoidance and distress, with a direct effect value of 0.28, accounting for 96.55% of the total effect.The total indirect effect of perspective taking on social avoidance and distress was significant, with the total indirect effect value of 0.01, accounting for 3.45% of the total effect. (4) In the intervention group, the perspective taking score of the post-test (15.69±3.86) was significantly higher than that of the pre-test (10.56±4.18) ( P<0.01). In the control group, there was no significant difference in the scores of perspective taking before and after the test((10.63±3.69), (11.44±3.03)) ( P>0.05). (5) In the intervention group, the scores of social avoidance and distress in the post-test (6.38±5.01) were significantly lower than that in the pre-test (15.00±6.07) ( P<0.001). In the control group, the score of social avoidance and distress in the post-test (15.81±6.33) was lower than that in the pre-test (19.00 ±5.38) ( P<0.05), but the scores of social avoidance and distress in the intervention group decreased even more. Conclusion:Perspective taking plays an mediating role between sub-threshold autistic traits and social avoidance and distress. Group counseling based on perspective taking was helpful to improve the perspective taking ability of people with high sub-threshold autistic traits and reduce social avoidance and distress behaviors.
RESUMO
Objective@#To further study the effects of distal-less homeobox gene 5 (Dlx-5) and Msh homeobox 1 (Msx-1) in the pathogenic mechanism of bisphosphonate related osteonecrosis of the jaw (BRONJ) .@*Methods@#Twenty-four SD rats were divided into two groups, the experimental group was injected intraperitoneally with zoledronic acid for 12 weeks (0.2 mg/kg, three times a week), and the control group was injected with saline solution for 12 weeks. The first mandibular molars were extracted after 12 weeks. All of the animals were sacrificed eight weeks after teeth extraction. The BRONJ was diagnosed by gross observation, X-ray examination and histopathlolgical examination. Through real-time PCR, the expression level of Dlx-5 and Msx-1 were detected in the mandible of BRONJ samples and normal samples.@*Results@#X-ray examination and histopathlolgical analysis showed the presence of BRONJ. The results of real-time PCR showed that the expression levels of Dlx-5 were increased (P=0.001) and the expression level of Msx-1 was decreased (P=0.001) in the experimental group compared with the control group.@*Conclusions@#Dlx-5 and Msx-1 genes play roles in the pathogenic mechanism of BRONJ.
RESUMO
Objective:To explore the directed forgetting ability of elder people to disease/death-related words under the conditions of the neutral and negative emotion.Methods:Thirty-six elder people and thirty-seven young people were included in this study.The directed forgetting effects to disease/death-related words were compared by using item-method directed forgetting paradigm under the neutral and negative emotional conditions.Results:Under the neutral and negative emotion conditions,higher recognition of to-be-remembered (TBR) than to-be-forgotten (TBF) words in elder group and younger group (Ps ＜ 0.05).The elder group showed the weaker effect of directed forgetting than younger group[neutral emotion:(0.4 ± 0.1) vs.(0.6 ± 0.1);negative emotion:(0.3 ± 0.1) vs.(0.7 ± 0.04),Ps ＜ 0.05].Under the negative emotion condition,elder people showed stronger effect of directed forgetting for sensitive words compared with control words[(0.4 ± 0.1) vs.(0.1 ± 0.1),P ＜ 0.001].Conclusion:The results suggest that the directed forgetting ability of elderly is weaker than that of young individuals,the ability of non-sensitive words than that with sensitive words,and the directed forgetting effect of elder people tonon-sensitive words in negative emotion is weaker than that in neutral emotion.
RESUMO
BACKGROUND:Bisphosphonates-related osteonecrosis of the jaw (BRONJ) is thought to be caused by trauma to dentoalveolar structures after dental extraction and implantation that have a limited capacity for bone healing followed by infection,suppuration and pain,further inducing equestration,but the pathogenesis is poorly understood.OBJECTIVE:To establish a stable animal model of BRONJ,and to observe its clinical manifestations,radiological changes and pathological characters,thereby paving ways for further understanding the pathogenesis of BRONJ.METHODS:Twenty-four female Sprague-Dawely rats were randomly allocated into experimental and control groups (n=12 per group).The rats in the experimental group were given the intraperitoneal injection of 0.2 mg/kg zoledronic acid,and the controls received the intraperitoneal injection of same volume of normal saline,three times weekly for consecutive 12 weeks.Subsequently,left first mandibular molars were extracted under general anesthesia,and the bone healing was observed.At 8 weeks after tooth extraction,all the rats were euthanized.The rat left jaws were examined by X-ray and micro-CT to explore the imaging changes.The histopathological characteristics of the mandibular soft and hard tissues were investigated by hematoxylin-eosin staining and Masson staining,to further testify the BRONJ model.RESULTS AND CONCLUSION:At 8 weeks after tooth extraction,the wound was unable to heal,and nonvital bone tissues were visible in the experimental group.The findings revealed on radiographies and pathological sections were in accordance with the characteristics of BRONJ.Masson staining results showed slender collagen fibrils around the dental extraction wound were slender in the experimental group.These results indicate that the intraperitoneal injection of zoledronate combined with tooth extraction can induce BRONJ in rats.The soft tissue toxicity and anti-angiogenesis of zoledronic acid can promote the occurrence of BRONJ,and the local acidic microenvironment caused by trauma probably is involved in the occurrence of BRONJ.
RESUMO
<p><b>OBJECTIVE</b>To observe the effect of different concentrations of zoledronic acid on human gingival fibroblast (HGF) cells proliferation and apoptosis, and to investigate the mechanism of bisphosphonate-related osteonecrosis of the jaw (BRONJ) caused by zoledronic acid.</p><p><b>METHODS</b>Different concentrations (0, 0.5, l.0, 5.0, 10.0 µmol/L) of zoledronic acid acted on the HGF. After 24 hours, flow cytometry was used to detect the rate of apoptosis and methyl thiazolyl terazolium (MTT) assay used to observe the proliferation of HGF. The effect of different concentrations of zoledronic acid on cell proliferation was examined by proliferation test on day 2, 4 and 7.</p><p><b>RESULTS</b>Flow cytometry showed that when the concentration of zoledronic acid was 0, 0.5, 1.0, 5.0, 10.0 µmol/L, the apoptosis rate was (0.67 ± 0.50)%, (2.13 ± 0.21)%, (3.27 ± 0.23)%, (4.17 ± 0.35)%, (9.87 ± 1.79)% respectively. The difference in HGF cells between control group and zoledronic acid groups was significant (P < 0.05) at three concentrations of zoledronic acid (1.0, 5.0, 10.0 µmol/L). The cell proliferation test showed an concentration-time dependent effect. With increase of concentration and time, the cell proliferation was significantly inhibited. There was significant difference in absorbance value among the different concentration groups (P < 0.05).</p><p><b>CONCLUSIONS</b>Zoledronic acid can inhibit HGF proliferation and promote its apoptosis.</p>
Assuntos
Humanos , Apoptose , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos , Conservadores da Densidade Óssea , Farmacologia , Proliferação de Células , Corantes , Difosfonatos , Farmacologia , Relação Dose-Resposta a Droga , Fibroblastos , Fisiologia , Citometria de Fluxo , Gengiva , Biologia Celular , Imidazóis , Farmacologia , Sais de Tetrazólio , Tiazóis , Fatores de TempoRESUMO
Objective To evaluate the safety,biocompatibility and efficacy of transcatheter closure of atrial septal defect(ASD)with no stainless-steel-screw occluder in canine model.Methods The device was constructed from superelastic Nitinol wires tightly woven into two flat disks and sewed with polyester fibers inside,with a pliable loop on the right-atrial-disk of the device,connecting to the delivery cable.ASD was created by transcatheter puncture and balloon dilatation and then closed by occluder under fluoroscopy in the catheterization laboratory.The location and the influence of the implanted device on function of tricuspid valve and mitral valve were evaluated by echocardiography.At 1,2,3 and 6 months after the operation,the animals were killed and autopsy was conducted.Results Eight dogs with puncture-produced ASD underwent ASD closing procedure successfully.The occluder showed no influence on the function of MV and AV demonstrated by echocardiogram.The two disks of the implanted device were covered with a smooth intact neogenesis layer in all dogs.Endocardial cells fully covered the surface of the two disk without inflammating reaction 3 months later. There was no evidence of corrosion on the surface of the nitinol wire removed from the dog after 6 months.Light microscopic examination of the liver,kidney,lung and spleen showed no evidence of embolization and inflammation.Conclusion Transcatheter ASD occlusion with new-type occluder is safe,feasible,effective and good biocompatibility with a good prospective clinical application.